Human P301L-Mutant Tau Expression in Mouse Entorhinal-Hippocampal Network Causes Tau Aggregation and Presynaptic Pathology but No Cognitive Deficits (original) (raw)
Figure 8
Biochemical detection of abnormal tau aggregation in EC-hTau mice.
(A, B) Levels of tau dimers (d) in the EC (A) and DG (B) of EC-hTau mice, NTG mice (negative control) and rTg4510 mice (positive control) were detected by western blot analysis with antibodies against total tau (Tau5). The middle panels show shorter exposures of the tau monomer (m) band, used for optical density quantification. GAPDH was used as a loading control. (C–D) Ratios of tau dimers to monomers as determined by densitometric quantitation of western blot signals obtained from EC (C) and DG (D) homogenates. ***p<0.0005 vs. all EC-hTau groups or *p<0.05 as indicated by brackets (Tukey test). Values are mean ± SEM. (E) Sarcosyl-insoluble tau was extracted from the EC and DG of EC-hTau and rTg4510 mice and detected by western blot analysis with the Tau5 antibody.