Human Nucleoporins Promote HIV-1 Docking at the Nuclear Pore, Nuclear Import and Integration (original) (raw)
Figure 2
All tested nucleoporins disrupt HIV-1 infectivity when depleted but have only a limited effect on MLV infection.
(A) The effect of nucleoporin knock-down on HIV-1 infectivity was assessed in P4-CCR5 indicator cells using a single cycle infectivity assay based on ß-galactosidase expression following infection with wild-type HIV-1 (8 or 4 ng) or (B) pseudotyped HIV-1 VSV-G (4 or 0.4 ng). Graphs show with log10 scale the mean luminescence values normalised for protein content +/− SEM and are each representative of 5 independent experiments. (C) Nup358/RanBP2, and Nup153 are specific for HIV-1 infection without effect on MLV infection. LV-shRNA transduced and control cells were infected with 5 ng of p24 of VSV-G pseudotyped NL4.3 luc virus and 5 ul of MLV luc/VSVG. Cell lysates were measured for luciferase activity 48 hr p.i. Luciferase values show averages of triplicate infections normalised by µg of proteins +/− SD.