Matrine Induces Apoptosis in Human Acute Myeloid Leukemia Cells via the Mitochondrial Pathway and Akt Inactivation (original) (raw)
Figure 2
Matrine induced apoptosis in AML cells.
A. AML cells, leukemic CD34+lin− cells, and normal CD34+lin− cells were treated with 1.5 g/L matrine for 24 h. The Annexin V-FITC binding and PI staining method was used to assess apoptosis, and representatives were shown. Both annexin V- and PI- negative (lower - left quadrant), annexin V- positive and PI- negative (lower - right quadrant), and both annexin V- and PI- positive (upper - right quadrant) cells were considered as the viable, early-phase apoptotic, late-phase apoptotic/necrotic cells, respectively. The percentage (%) of cells was described in each quadrant. B. AML cells were treated with various concentrations of matrine (0∼2 g/L) for 24 h. C. AML cells were treated by 1.5 g/L matrine for 12 h, 24 h and 48 h. The values represent the mean ± SD of at least three independent experiments. *P<0.05 vs the respective control.