Matrine Induces Apoptosis in Human Acute Myeloid Leukemia Cells via the Mitochondrial Pathway and Akt Inactivation (original) (raw)
Figure 5
Effects of matrine on the activated caspase-3 in AML cells for 24 h. A.
The expression of activated caspase-3 was analyzed by FCM and representatives were shown in AML cells treated by 1.5 g/L martrine for 24 h. B. AML cells were treated by various concentrations of matrine. C. Effects of Z-DEVD-FMK on matrine-induced apoptosis detected by annexin V-FITC binding and PI staining method. Apoptotic cells were determined after treatment with matrine (1.5 g/L) in the presence (+) or absence (−) of Z-DEVD-FMK (5 µM) for 24 h. The values represent the mean ± SD of at least three independent experiments. *P<0.05 vs the respective control, #P<0.05 vs the matrine only group.