Anion-Sensitive Fluorophore Identifies the Drosophila Swell-Activated Chloride Channel in a Genome-Wide RNA Interference Screen (original) (raw)
Figure 8
Chimeras between dBest1 and mBest2 confer swell activation on mBest2.
(A) Truncation of dBest1 does not interfere with swell sensitivity. Constitutive currents are apparent with both dBest1 and Stop 383 overexpression in iso-osmotic solutions (320 mOSM). With swell, current increased dramatically for both constructs (Student’s t-test, * p<0.05, ** p<0.005). (B) d94m current increases significantly within the first 2 min of swelling (* Student’s t-test, p<0.05). (C) Time course for d64m swell activation. (D) Current-voltage relations for constitutive d64m, and following 2 min 200 mOSM solution. (E) Selectivity sequence for the constitutively active d64m current. (F) Selectivity sequence for the constitutively active mBest2 currents.