Routine Habitat Change: A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice (original) (raw)
Figure 1
Analysis of stool microbiota changes following husbandry changes.
A) Timeline of experimental approach. Fresh fecal samples were collected daily at each time point from individual mice (downward arrows). Day 0 denotes baseline samples obtained just prior to transfer from an SPF facility to a conventional housing facility (upward block arrow). Upon transfer, either bedding (group 1) or food (group 2) was changed (from corncob to Carefresh bedding, and Teklad 2918 to LabDiet 5058, respectively). Control mice (group 3) were also transferred and placed in a fresh cage, but maintained on the same type of food and bedding (corncob and Teklad 2918). Day 1 is 24 hours after the combined facility transfer/cage change for all mice. B) Principal coordinate analysis of Eubacterial-TRFLP data. Each data point represents a single mouse specimen at days 0 (yellow squares), 1 (black circles) and 5 (blue triangles) from mice in three experimental groups: bedding change (n = 6), food change (n = 8) and control (n = 8). A PCoA was performed using all data, and then graphed for each of the three experimental groups separately.