Routine Habitat Change: A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice (original) (raw)
Figure 3
Eubacterial-TRFLP analysis of fecal microbiota in mice following a combined facility/cage change.
A) Taxonomic plot of bacterial profiles daily from day 0 to day 5. A facility/cage change occurred on day 0 after stool was sampled (see Fig. 1A for timeline). DNA isolated from the feces of each mouse in the control group (group 3, n = 8) was analyzed by TRFLP as described in the methods. Mice A, B, G, H were males; C, D, E, F were females. Note that over time, the group of Lactobacillus initially lost at day 1, returns to the population profile in 5 of 8 mice by day 4, and 7 of 8 mice by day 5. B) Principal coordinate analysis of TRFLP data for control mice over time. Each data point represents a single mouse specimen at days 0 (pink circles), 1 (black squares), 2 (blue inverted triangles), 3 (green inverted triangles), 4 (orange triangles) and 5 (yellow triangles) from mice in the control group analyzed in A. Note, the shift and clustering of data at day 1, gradually returns to resemble the original pattern by days 4 and 5.