Hypoxia-Induced Modulation of Apoptosis and BCL-2 Family Proteins in Different Cancer Cell Types (original) (raw)
Figure 4
Effect of hypoxia, etoposide and paclitaxel on the mRNA expression level of genes involved in the apoptotic pathway.
Results were obtained using “TLDA Human Apoptosis Panel” (Applied Biosystems) (TLDA). HepG2, A549, MDA-MB231 and Hep3B cells were incubated 16 hours under normoxia (N, 21% O2) or hypoxia (H, 1% O2) in the presence or not of etoposide (E, 100 µM in Hep3B cells and 50 µM in the other cell types) or paclitaxel (T, 10 µM) in HepG2 cells. After incubation, total RNA was extracted, submitted to reverse transcription and then to TLDA analysis. 18S was used as housekeeping gene for data normalization. Actual numerical values are provided in Table S2. Genes shown in bold are genes whose expression was validated by qRT-PCT.