The Balance of Apoptotic and Necrotic Cell Death in Mycobacterium tuberculosis Infected Macrophages Is Not Dependent on Bacterial Virulence (original) (raw)
Figure 1
Polycaspase activation in M. tuberculosis infected RAW macrophages over 72 hours.
RAW macrophages were infected with M. tuberculosis GC1237 wild-type and trafficking mutant strains Tn::moaC1 and Tn::Rv1503c. After 24, 48 and 72 hours, nuclei were stained with Hoechst stain (blue), and active caspases stained with fluorescent polycaspase inhibitor (FLICA; red), and the cells analysed by automated confocal microscopy. Caspase staining (A) (surface of caspase/number of nuclei) and number of nuclei (B) were measured using Image Mining Software developed at the Institut Pasteur, Korea. Bar graphs are Mean+SEM of triplicate samples. *p = <0.05, **p = <0.01, ***p = <0.001 by 2-way ANOVA, with the upper and lower p values for GC1237 vs Tn::moaC1 and Tn::Rv1503c respectively. (C) Representative microscopy at 72 hours is shown. Results are representative of 3 independent experiments with similar results.