E4orf1 Improves Lipid and Glucose Metabolism in Hepatocytes: A Template to Improve Steatosis & Hyperglycemia (original) (raw)
Figure 1
E4orf1 suppresses glucose output in the presence of gluconeogenic stimulators in HepG2.
(A) Western blot showing Ad36 E4orf1 protein expression in HepG2 cells following transfection with V5-Ad36 E4orf1 plasmid DNA. (B) Glucose output by HepG2 cells following E4orf1 or null Vector transfection. Cells were stimulated with gluconeogenic cAMP (1 mM) (C) and Dexamethasone (500 nM) (D) in the absence or presence of insulin (I). Expressed as mean mM glucose per mg of protein, ± SD. E4orf1 significantly suppresses glucose output in the basal and insulin stimulated conditions (p = 0.0001 and 0.003, respectively).