Establishment and Characterization of a Highly Tumourigenic and Cancer Stem Cell Enriched Pancreatic Cancer Cell Line as a Well Defined Model System (original) (raw)

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Figure 11

Expression of ALDH1 and CD133 in primary and xenograft tumours.

Immunohistochemistry of ALDH1 and CD133 was performed for primary and xenograft tumours. A–C. ALDH1 expression was dispersed in primary (A) and more defined in xenograft tumours (B, C). Cells expressing ALDH1 were typically arranged in ductal structures with focal mucin inclusions (B). Ducts lined with cells not expressing ALDH1 lacked these inclusions (indicated by asterisks in A and C). Panel C shows ALDH1-positive cells invading a lymphatic vessel, while surrounding tumour cells were ALDH1-negative. Normal pancreatic tissue was present (Picture C, indicated by „N”). E,F. Expression of CD133 was observed only in xenograft tissues. Here, CD133 was expressed predominantly in the lumen of ducts. Ductal epithial cells stained positive at the apical membrane (E) and occasionally intracellularly (F, arrow heads). Detritus within the lumen was typically stained positive.

Figure 11

doi: https://doi.org/10.1371/journal.pone.0048503.g011