Inhibition of Tankyrases Induces Axin Stabilization and Blocks Wnt Signalling in Breast Cancer Cells (original) (raw)
Figure 1
XAV939, a chemical inhibitor of tankyrases, stabilizes Axin in breast cancer cells.
(A–C) Human MDA-MB-231 and MCF-7 cells and mouse EMT6 cells were treated overnight with 10 µM XAV939 in the presence or in the absence of Wnt3a conditioned medium either overnight or for 4 h and protein levels of Axin1, Axin2 and tankyrases (TNKS) were determined by immunoblotting of total cell lysates. (D) MDA-MB-231 cells were treated overnight with 10 µM XAV939 and IWR-1-endo and after 4 h incubation with Wnt3a conditioned medium, cells were lysed and subjected to immunoblot analysis using anti-Axin1, anti-Axin2 and anti-Tankyrase antibodies. All samples were also probed with anti-actin antibody to verify equal protein loading.