Cohesion Fatigue Explains Why Pharmacological Inhibition of the APC/C Induces a Spindle Checkpoint-Dependent Mitotic Arrest (original) (raw)
Figure 2
proTAME induces SAC re-activation following loss of sister chromatid cohesion.
(A, B) Graphs measuring cyclin B fluorescence intensity upon proTAME treatment (5 µM), either in the absence (A) or presence (B) of AZ3146. In each case, T = 0 was normalised to the onset of metaphase or prometaphase respectively. Arrows indicate the time at which anaphase or cohesion fatigue occurs. Data from one representative cell is shown for each treatment.