Cohesion Fatigue Explains Why Pharmacological Inhibition of the APC/C Induces a Spindle Checkpoint-Dependent Mitotic Arrest (original) (raw)
Figure 3
Increasing the level of cohesin on the chromosomes inhibits the mitotic arrest caused by proTAME.
(A) Immunoblot of HeLa cells treated showing Wapl depletion upon RNAi treatment. Bub3 was used as a loading control. (B) Immunofluorescence images of mitotic HeLa cells showing an increased amount of the cohesin subunit Smc3 on the chromosomes upon Wapl depletion. (C) Scatter plot of HeLa cells depleted of Wapl and treated with proTAME, showing an increase in the amount of time cells take to undergo cohesion fatigue. P values were calculated using two-tailed Mann-Whitney test. (D) Cell fate profiles of HeLa cells depleted of Wapl and treated with different concentrations of proTAME, as described in Fig. 1A. Each line represents an individual cell and different colours represent the different cell fates (see legend).