New Alternately Colored FRET Sensors for Simultaneous Monitoring of Zn2+ in Multiple Cellular Locations (original) (raw)
Figure 1
Nuclear Localization and Nuclear Exclusion Signal Sequence constructs.
A NLS and NES were cloned into pcDNA 3.1 (+) vector upstream _Bam_H I. A) Schematic of FRET sensor construct. B) Representative images of transfected sensor showing localization to either the nucleus or cytosol. Scale bar = 20 µm.