Acidic Nanoparticles Are Trafficked to Lysosomes and Restore an Acidic Lysosomal pH and Degradative Function to Compromised ARPE-19 Cells (original) (raw)
Figure 2
Rapid delivery of nanoparticles to lysosomes.
A. Composite image of ARPE-19 cells with internalized NP3R nanoparticles (red, 1 mg/ml) and Lysotracker (green) after 15 min incubation. B. NP3R and lysosomes after 30 min incubation. C. NP3R and lysosomes after 1 hr. incubation. Bar = 10 µM in panels A-C. D. Colocalization of NP3R nanoparticles with ARPE-19 cells as a function of time. Each point is the mean +/− standard deviation of the Pearson’s coefficient in one microscope field; n = 3. Error bars are present but too small to be detected for the 15 min point.