Acidic Nanoparticles Are Trafficked to Lysosomes and Restore an Acidic Lysosomal pH and Degradative Function to Compromised ARPE-19 Cells (original) (raw)
Figure 5
Nanoparticles reduce autofluorescence and opsin levels associated with ingestion of photoreceptor outer segments.
A. Sample readout of the FACS analysis demonstrating treatment with NP3 greatly reduced the mean autofluorescence at 488 nm in RPE cells treated with chloroquine (CHQ) and phagocytosed photoreceptor outer segments (POS). B. Nanoparticles reduced the autofluorescence in ARPE-19 cells given POS, CHQ, and or POS+CHQ. Bars represent the mean ± SEM of autofluorescence detected at 488 nm. * p<0.05 vs. control; # p<0.05 vs. POS+CHQ. ANOVA. C. Immunoblot for opsin detected in ARPE-19 cells in the absence of photoreceptor outer segments (Control), after exposure to outer segments over 7 days (POS), and with a delayed addition of PLA NP3 after each outer segment feeding (POS+NP3). The blot was at the predicted size of ∼40 kDa. GAPDH binding of the blot is demonstrated below. D. Quantitation of opsin levels in immunoblots. Levels were first controlled for GAPDH staining, and then normalized to the mean POS value in each blot to control for variation. * p<0.001, n = 4.