Withaferin A Induces Proteasome Inhibition, Endoplasmic Reticulum Stress, the Heat Shock Response and Acquisition of Thermotolerance (original) (raw)
Figure 5
Effect of mild heat shock on WA-induced BiP, GRP94, HSP30 and HSP70 accumulation.
A) Cells were exposed to 2 or 5 µM WA at 22°C or 30°C for 8 h. Following treatment, cells were harvested and total protein was subjected to immunoblot analysis. B & C) Image J software was used to perform densitometric analysis of the signal intensity for BiP, GRP94, HSP30 and HSP70 protein bands of western blot images as described in Materials and methods and in the legend for Figure 3. The data are expressed for each treatment as a ratio to control levels (for BiP and GRP94 accumulation) or as percentage of the maximum signal (combined WA and mild heat shock treatment for HSP30 and HSP70 accumulation). Significant differences between the control cells and treated cells are indicated as * (p<0.05). These data are representative of three separate experiments.