Withaferin A Induces Proteasome Inhibition, Endoplasmic Reticulum Stress, the Heat Shock Response and Acquisition of Thermotolerance (original) (raw)
Figure 6
Intracellular localization of BiP in response to A23187, WA and MG132.
Cells were grown on glass coverslips and were treated with 5 µM WA for 16 h, 7 µM A23187 or 30 µM MG132 for 24 h at 22°C. BiP was indirectly detected with an anti-BiP antibody and a secondary antibody conjugated to Alexa-488 (green). Actin and nuclei were stained directly with phalloidin conjugated to TRITC (red) and with DAPI (blue), respectively. From left to right the columns display fluorescence detection channels for actin, BiP and merger of actin, DAPI and BiP. The white arrows indicate perinuclear localization while the yellow arrows show the presence of BiP near the cell membrane. The 20-µm white scale bars are indicated at the bottom right section of each panel. These results are representative of 3 different experiments.