Withaferin A Induces Proteasome Inhibition, Endoplasmic Reticulum Stress, the Heat Shock Response and Acquisition of Thermotolerance (original) (raw)
Figure 7
Localization of WA-induced HSP30 accumulation.
Cells were cultured on glass coverslips and incubated with 5 µM WA for 8 or 18 h or 30 µM MG132 for 24 h at 22°C. HSP30 was indirectly detected with an anti-HSP30 antibody and a secondary antibody conjugated to Alexa-488 (green). Actin and nuclei were stained directly with phalloidin conjugated to TRITC (red) and with DAPI (blue), respectively. In the 18 h set of images (bottom row), the white arrows indicate examples of structures that display co-localization of both actin and HSP30. The 5 or 20 µM white scale bars are indicated at the bottom right section of each panel. These data are representative of three separate experiments.