Arresten, a Collagen-Derived Angiogenesis Inhibitor, Suppresses Invasion of Squamous Cell Carcinoma (original) (raw)
Figure 4
Arresten promotes an epithelial morphology of HSC-3 cells and increases the amount E-cadherin in cell-cell contacts.
A. Arresten overexpression induced a cobblestone-like appearance in HSC-3 tongue squamous cell carcinoma cells (200×magnification). B. Immunostaining of E-cadherin (red) in cultured Ctrl-HSC and Arr-HSC cells (blue, DAPI). Scale bar 10 µm. C. 10 µg of total protein from lysed cell extracts was analyzed by Western blotting with E-cadherin antibody. β-actin was used as a loading control. D. The relative band intensities were quantified (n = 3 Western analyses from separate protein extractions; mean ± SEM). E. mRNA expression of E-cadherin in cultured Ctrl-HSC (N = 6, n = 12) and Arr-HSC (N = 3, n = 6) cells (N = number of clones analyzed; n = number of samples analyzed). The expression levels were normalized to that of the GAPDH housekeeping gene and are presented relative to values obtained for Ctrl-cells (mean ± SEM) Students t-test, ***p<0.001, *p<0.05.