Neutrophil-Derived Myeloperoxidase Aggravates Non-Alcoholic Steatohepatitis in Low-Density Lipoprotein Receptor-Deficient Mice (original) (raw)
Figure 5
General reduction of diet-induced hepatic inflammation in _LDLR_−/−/_MPO_−/−tp mice.
A) Significantly lower number of hepatic Ly-6G+ neutrophils and CD3+ T-lymphocytes in _LDLR_−/−/_MPO_−/−tp mice as compared with _LDLR_−/−/MPO+/+tp mice after 8 weeks of high-fat feeding (Ly-6G: 36.7±2.6 vs. 47.8±3.1 cells/mm2, p = 0.03; CD3: 49.1±4.2 vs. 62.9±5.0 cells/mm2, p = 0.04). Pictures represent examples of the stainings (200× magnification). B) Hepatic pro-inflammatory cytokine/chemokine expression is substantially reduced in _LDLR_−/−/_MPO_−/−tp mice after 8 weeks high-fat diet (TNF-α 1.8-fold, p = 0.03, IL-1α 1.6-fold, p<0.01, IL-6 1.3-fold, p = 0.67, Mcp-1 2.5-fold, p<0.01), in parallel with a reduction of CD68 expression (1.3-fold, p<0.05).