Antigen Recognition By Autoreactive Cd4+ Thymocytes Drives Homeostasis Of The Thymic Medulla (original) (raw)
Figure 5
Roles of LTβR, RANK and CD40 signaling in mTEC expansion and maturation.
2-dGUO-treated WT embryonic thymic lobes were cultured for 4 days in medium containing agonistic anti-LTβR antibodies, CD40L and/or RANKL. Control cultures were un-supplemented (medium) or not treated with 2-dGUO. (A) Representative FACS profiles are shown for Ly51 expression by CD45−EpCAM+ TECs (top) and I-Ab and CD80 expression by CD45−EpCAM+Ly51−/lo mTECs (bottom) for the indicated cultures: percentages of cells are indicated. (B) Graphs show mTEC numbers (left) and frequencies of mature I-AbhiCD80hi mTECs (right) for the indicated conditions: data from 3 experiments; lines represent medians.