PDGF is Required for Remyelination-Promoting IgM Stimulation of Oligodendrocyte Progenitor Cell Proliferation (original) (raw)
Figure 4
Synergistic inhibition of apoptotic signaling in OPCs by rHIgM22 and PDGF/FGF-2. A+B:
OLs were cultured for 7 days on fibronectin with rHIgM22 (5 µg/ml) or isotype-control IgM (IC) (5 µg/ml) in the absence or presence of growth factors PDGF-AA and FGF-2. A: Representative Western blots of cleaved caspase-3 (19 kDa fragment), cleaved caspase-9 (17 kDa fragment) and β-actin from OLs cultured for 1–7 days on fibronectin with rHIgM22 (5 µg/ml) or isotype-control HIgM (IC IgM) (5 µg/ml) in the absence or presence of growth factors PDGF-AA and FGF-2. B: Quantitative analysis from 3 independent experiments under the conditions described above. Background is subtracted from each value and normalized against β-actin. Data are presented as mean ± S.D. (n = 3). *p<0.05 compared to controls.