Triggering of Inflammasome by Aggregated α–Synuclein, an Inflammatory Response in Synucleinopathies (original) (raw)
Figure 1
IL-1β synthesis and secretion in human monocytes induced by fibrillar αSyn.
(A) Monocytes were exposed for 2, 6 and 18 h to 40 nM αSyn F or 1 µg/ml LPS (positive control) or vehicle (saline) and the expression of pro-IL-1β was evaluated by real-time PCR. Real time data are shown as the mean ± S.D. of results obtained with cell preparations from 5 different donors; experiments with each cell preparation were conducted in duplicate. **p<0.01, ***p<0.001. (B) IL-1β synthesis was also evaluated by immunoblot analysis in cell lysates from monocytes treated as in A. The immunoblot is from one representative donor. (C) Culture supernatants from monocytes that had been harvested for quantification of mRNA levels, reported in A, were collected and evaluated for their IL-1β content by ELISA. Results are the mean ± S.D. of 5 determinations made in duplicate with different cell preparations derived from different donors. ***p<0.001 (D) Culture supernatants of monocytes, harvested for the evaluation of IL-1β synthesis, as in B, were collected and the released IL-1β revealed in immunoblot.