The Effects of Oral and Enteric Campylobacter concisus Strains on Expression of TLR4, MD-2, TLR2, TLR5 and COX-2 in HT-29 Cells (original) (raw)
Figure 8
Production of IL-8 by HT-29 cells induced by C. concisus strains.
A. HT-29 cells were incubated with C. concisus strains at a multiplicity of infection (MOI) of 100 for 24 hours. Concentrations of IL-8 in the cell culture supernatants were measured by ELISA. The basal production of IL-8 (HT-29 cells without C. concisus infection) has been subtracted from values shown in Figure 8A. Data were the average of triplicates ± standard error. The level of IL-8 induced by C. concisus strains from patients with IBD was not statistically different from that induced by C. concisus strains from the healthy controls (_P_>0.05). Five C. concisus strains (H2O1-H6O1) were isolated from healthy controls; the remaining nine strains were from patients with IBD. B. HT-29 cells were incubated with three C. concisus strains respectively at four different MOIs (25, 50, 100 and 200) for 24 hours. The three C. concisus strains used were P1CDO2 (blue), P1CDO3 (red) and P1CDB1 (green). Concentrations of IL-8 in the cell culture supernatants were measured by ELISA. The basal production of IL-8 (HT-29 cells without C. concisus infection) has been subtracted from values shown in Figure 8B. Data were the average of triplicates ± standard error.