A Novel Anti-Inflammatory and Pro-Resolving Role for Resolvin D1 in Acute Cigarette Smoke-Induced Lung Inflammation (original) (raw)
Figure 7
RvD1 enhances macrophage phagocytosis in vivo and ex vivo.
Mice were exposed to air or CS with or without RvD1 as described. Lungs were digested and macrophages were isolated as described in Methods. The percentage of F4/80/Gr-1 double positive cells was quantified by flow cytometry. (A) Dot plots shown are from one vehicle control mouse and two RvD1-treated mice. (B) BAL cells were harvested from naïve mice and incubated ex vivo with 1 ng/ml IL-1β and 100 nM RvD1 for 30 minutes prior to the addition of FITC-labeled latex beads. Percentage of F4/80+ macrophages that ingested FITC-latex beads was quantified by flow cytometry. Representative histogram of one out of 6 mice is shown. (C) Mean ± SEM is shown for 6 animals from two independent experiments. *p<0.05, **p<0.01, ***p<0.001, Student’s t-test.