OstemiR: A Novel Panel of MicroRNA Biomarkers in Osteoblastic and Osteocytic Differentiation from Mesencymal Stem Cells (original) (raw)
Figure 5
Quantification of miRNA expression levels during osteocytic differentiation.
(A) Expression of internal controls for miRNA quantification. Snord85, Snord66 and Rnu6 levels during osteogenesis of KUSA-A1 were quantified in PCR array. miRTC, miRNA reverse transcription control. PPC, positive PCR control. (B–K) Quantification of miRNA expression levels by using miRNA array (B, E, G, I, K) or qRT-PCR (C, D, F, H, J). Values were normalized to snord66 levels (C, D, F, H, J). *, P<0.05 (n = 4). n.s., not significant.