Real-Time Motion Analysis Reveals Cell Directionality as an Indicator of Breast Cancer Progression (original) (raw)
Figure 3
Cell lines of the MCF10A series show distinct responses to EGF and LPA.
(A–D) M1–M4 cells were stimulated with 5 ng/mL EGF (red) or 1 µM LPA (blue) and perturbations of average speed and of directionality (angle distributions and CV) compared to controls (black) were assessed (mean ± SD). Rose plots depict controls (unfilled, black bars) and 5 ng/mL EGF (filled, red bars) or 1 µM LPA (filled, blue bars). Statistical significance: * p<0.05, ** p<0.01, *** p<0.001 (Tukey-Kramer test, n = 3 for all conditions except M2 with EGF where n = 2). All comparisons were made with M1 cells unless indicated by pairing-brackets.