Real-Time Motion Analysis Reveals Cell Directionality as an Indicator of Breast Cancer Progression (original) (raw)
Figure 5
The migratory phenotype of MDA-MB 231T is similar to that of M4 cells.
(A) Phase contrast images of MDA-MB-231T cells moving into open space after 12 h under control (Black), 5 ng/mL EGF (red), and 1 µM LPA (blue) treatments. Bar = 100 µm. (B) Right: The effects of EGF and LPA treatment on average speed (top) and directionality, CV (bottom) determined over all times and space, were compiled from 5–6 independent experiments and reported as mean ± SD. Left: Aggregate directionality profiles for control, EGF and LPA conditions. Statistical significance: *p<0.05 (Tukey-Kramer test, n = 3). (C) Representative spatiotemporal heat plots show speed responses in control, EGF, and LPA treated cells. See Fig. 4 for details.