Oleuropein Aglycone Protects Transgenic C. elegans Strains Expressing Aβ42 by Reducing Plaque Load and Motor Deficit (original) (raw)
Figure 1
Effect of OLE on Aβ-induced paralysis in CL2006 transgenic C. elegans strain.
(A) Diagram illustrating the paralysis assay showing when the drug was administered and when the paralysis assay was scored. (B–C) Dose-response effect of OLE on the paralysis induced by Aβ expression in CL2006 and CL802 transgenic worms. Egg-synchronized worms were placed at 16 °C on fresh NGM plates seeded with OP50 E. coli and, at L2 (B) or L3 (C) stage, were fed with OLE (12.5–500 µM). The number of paralyzed worms was scored 48 h or 24 h after treatment (at L4 larval stage) for L2- and L3-treated worms, respectively. Data are shown as percentage±SE of paralyzed worms to vehicle treated ones (n = 100 worms/group, 3 independent assays). (D) Percentage of paralyzed worms fed with OLE. CL2006, CL802 and N2 worms, cultured as above, were fed 50 µM OLE at L1 or L2 and 500 µM at L3. Tetracycline at 50 µM was administered at L3 as positive control. The number of paralyzed worms was scored at L4 larval stage. Data are shown as percentage±SD of paralyzed worms to vehicle treated ones (n = 100 worms/group, 3 independent assays). °° p<0.01 vs. CL802, **p< 0.01 vs CL2006 worms fed with vehicle (One-way ANOVA test), and +p<0.01 vs. CL2006 worms fed with 50 µM OLE at L2 (Student’s t test).