Genetic Activation of Nrf2 Protects against Fasting-Induced Oxidative Stress in Livers of Mice (original) (raw)
Figure 4
Oxidative stress and glutathione (GSH) contents in mouse livers.
(A) Detection of superoxide in liver. Frozen liver tissue was cut into 6 µM sections and stained with dihydrothidium (DHE). (B) Contents of lipid peroxidation products (represented by malonaldehyde equivalent) and the reductive GSH in livers of mice possessing different Nrf2 activity. Data are presented as mean ± S.E. (n = 5). Two-way ANOVA was performed with genotype and treatment as main factors. Significant main effects and interactions were followed by Student-Newman-Keuls comparisons to assess the differences between groups. Asterisks (*) represent statistical differences (p<0.05) compared with WT mice on the same treatment. Pounds (#) represent statistical differences (p<0.05) between fasted and control mice of the same genotype.