Derivation and Expansion Using Only Small Molecules of Human Neural Progenitors for Neurodegenerative Disease Modeling (original) (raw)
Figure 2
Neural epithelial cells can be respecified along both the dorsoventral and rostrocaudal axes.
(A) qRT-PCR analysis for the indicated marker on neural epithelial cells cultured under the indicated conditions for 6 days. Error bars represent the standard deviation from 3 independent cultures. (B) Immunostaining of neural epithelial cells cultured with the indicated concentration of PMA or CHIR for 6 days for PAX3, NKX2.2, NKX6.1, FOXA2, and SOX1. (C) qRT-PCR analysis of neural epithelial cells cultured with 1 µM RA for 1 or 8 days for the indicated rostrocaudal marker. Error bars represent the variation from 2 independent cultures. Scale bars are 100 µm. See also Figure S3.