Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes (original) (raw)
Figure 5
WGA and allele-specific PCR analysis.
(A) Allele-specific PCR with primers for the D21S11 and D21S1435 markers on WGA-DNA and genomic-DNA from trisomic cells yielded the same allele profiles. (B) Capillary electrophoresis in a Bioanalyzer of the PCR products for D21S11revealed the presence of two different alleles in two microdissected chromosome pairs. (C) PCRs with primers for the D21S1435 marker produced fragments of ∼200 bp in a 1.5% agarose gel (upper panel). Capillary electrophoresis in a Bioanalyzer revealed the presence of two alleles within this band (181 bp and 189 bp) in a limited number of microdissected chromosome pairs (lower panel).