Genetic and Biochemical Characterization of the MinC-FtsZ Interaction in Bacillus subtilis (original) (raw)
Figure 4
Electron microscopy of FtsZ polymers formed in the presence or absence of MinC.
Purified wild-type and mutant FtsZ were polymerized in reactions containing 5 µM of FtsZ, Mes/NaOH 50 mM, MgCl2 10 mM, KCl 133 mM, pH 6.5, and either no (top row) or 20 µM MinC (bottom row). Polymerization reactions were spotted on carbon coated 400 mesh copper grids and stained with uranyl acetate for transmission electron microscopy. Scale bars equal 200 nm.