Human S100A9 Protein Is Stabilized by Inflammatory Stimuli via the Formation of Proteolytically-Resistant Homodimers (original) (raw)
Figure 4
hS100A9 protein was unstable in LEP cells but could be rescued by MG132 and hS100A8.
hS100A8 and hS100A9 expression vectors were transfected into human fibroblasts (LEP cells) as described before for COS cells. After SDS-PAGE and Western blots were performed either with (A) anti-hS100A9 or (B) anti-hS100A8. Lane 1 (NT) represented non-transfected cells; from lane 2 to lane 4 cells were transfected with hS100A9 (panel A; A9 T) or with hS100A8 (panel B; A8 T); in lane 5 (A8/A9 T) cells were co-transfected with both constructs.