Human S100A9 Protein Is Stabilized by Inflammatory Stimuli via the Formation of Proteolytically-Resistant Homodimers (original) (raw)
Figure 6
LPS and IL1β induced hS100A9 protein stabilization.
COS cells were transfected either with hS100A8 or hS100A9 constructs, as described above. 24 h after transfection, COS cells were stimulated with either 1 or 10 ng/ml IL1β or alternatively with either 10 or 100 ng/ml LPS. Samples were collected and analyzed by Western blot. Filters were stained with (A) anti-hS100A9 and (B) anti-hS100A8. Lane 1 (NT) represented non-transfected cells; from lane 2 to 6, COS cells were transfected with (A; A9 T) hS100A9 or (B; A8 T) hS100A8 separately; in lane 7 (A8/A9 T) COS cells were co-transfected with both plasmids.