Inhibition of Enveloped Viruses Infectivity by Curcumin (original) (raw)
Figure 1
Treatment of curcumin reduces infectivity of enveloped viruses.
(A) 4 HA units of Newcastle disease viruses (NDV) were incubated with 2-fold serially diluted curcumin or DMSO (vehicle control) and the hemagglutination inhibitory activity of curcumin was tested by incubation with chicken RBC at room temperature for 30 minutes. (B) time-of-drug addition test: 10 µM of curcumin or DMSO (as solvent control) was included into culture medium at various time points of Japanese encephalitis virus (JEV) or Dengue virus (DV-II) infection (200 pfu), for instance: (1) full time treatment: curcumin was added to vero cells at 8-hour prior to infection and included throughout the time of infection, (2) co-treatment: curcumin mixed with virus in the infection medium was added simultaneously to the cells and left on the cells throughout; (3) after-entry: curcumin was added to cells at 2 hpi and remained throughout the time of infection. Small-sized plaque of DV-II was indicated by arrowhead. Consistent results were observed from at least three independent experiments.