DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples (original) (raw)
Figure 1
Intra- and inter-method accuracy and precision.
Distribution of DNA sample concentration (dispersion chart) was estimated by both NanoDrop (black) and Qubit (gray) on repeated (n = 20) measurements of two commercial human genomic DNA preparations (Sample L 200 ng/µl; Sample G 5 ng/µl). For both samples, NanoDrop overestimated the DNA concentration (+8.8% for L and +24.0% for G, p<0.0003), while Qubit underestimated it (−5.0% for L and −7.3% for G, p<0.005).