HnRNP A1/A2 and SF2/ASF Regulate Alternative Splicing of Interferon Regulatory Factor-3 and Affect Immunomodulatory Functions in Human Non-Small Cell Lung Cancer Cells (original) (raw)
Figure 4
Minigene splicing assay of IRF-3.
(A) The wild type (wt) and mutant (mu) versions of the IRF-3 minigene are shown. PCMV, promoter of the pcDNA3.0 vector. pA, polyA signal. IRF-3 exons from 1 to 4 are numbered. The black solid line represents introns. (B) Transient transfection of wt-IRF-3 or mu-IRF-3 minigenes was performed in A549 cells and the relative expression levels of the IRF-3 isoforms were measured by RT-PCR analysis. (C) The graph indicates the ratio isoform FL/(FL+I+II) and the values are mean ± SD for n = 3 experiments. #P and ##P<0.05 compared to wt-IRF-3 minigene transfection.