HnRNP A1/A2 and SF2/ASF Regulate Alternative Splicing of Interferon Regulatory Factor-3 and Affect Immunomodulatory Functions in Human Non-Small Cell Lung Cancer Cells (original) (raw)
Figure 5
Depletion of hnRNP A1/A2 or SF2/ASF reduces IFNβ and IP-10 expression in human NSCLC cells.
A549 and Calu-6 cells were performed specific siRNA-mediated knockdown of hnRNP A1/A2 or SF2/ASF. Mock, cells transfected with the mismatched control siRNA. Control, cells without specific or mock siRNA transfection and Poly(I:C) stimulation. (A) After siRNA transfection and subsequent Poly(I:C) stimulation, the mRNA expression of IFNβ and IP-10 genes was analyzed by semi-quantitative RT-PCR. Total RNA isolated from A549 cells without reverse transcription was used as negative control. (B) Secretion of IFNβ (top) and IP-10 (bottom) proteins was examined by ELISA assay. The value for each assay is presented as mean ± S.D. for three independent experiments. #P and ##P<0.05 compared to mock-transfected A549 and Calu-6 cells, respectively.