HnRNP A1/A2 and SF2/ASF Regulate Alternative Splicing of Interferon Regulatory Factor-3 and Affect Immunomodulatory Functions in Human Non-Small Cell Lung Cancer Cells (original) (raw)
Figure 6
Depletion of hnRNP A1/A2 or SF2/ASF mediates proinflammatory effects as detected in A549 cell/PBMC co-cultures.
To explore the overall regulatory potential of A549 cells with specific siRNA-mediated hnRNP A1/A2 or SF2/ASF depletion on inflammatory cytokine production, co-cultivation with human PBMC was performed in the presence of PHA. Control, A549 cells cultured without PBMC. Mock, A549 cells transfected with the mismatched control siRNA. The operational approach of A549 cell/PBMC co-culture was performed as described in Materials and Methods section. After 72 h, co-culture supernatants were collected and production of TNF-α (A) and IL-10 (B) was examined by ELISA assay. Data are shown as mean ± S.D. for three independent experiments. #P and ##P<0.05 compared to co-cultures with mock-transfected cells.