Elevated Protein Carbonylation, and Misfolding in Sciatic Nerve from db/db and Sod1−/− Mice: Plausible Link between Oxidative Stress and Demyelination (original) (raw)
Figure 6
In vitro assessment of oxidative stress-induced aggregation of PMP22 protein.
Purified PMP22 protein was subjected to in vitro oxidation with increasing concentrations of tert-butyl hydroperoxide. SDS-PAGE was performed on oxidized PMP22 protein from both the soluble and detergent-soluble protein fractions. Results are expressed as mean ± SEM (n = 6; *p<0.05 by two-tailed t-test).