Gauging NOTCH1 Activation in Cancer Using Immunohistochemistry (original) (raw)
Figure 2
NICD1 staining in an archival human T-LL of known genotype.
A) Identification of activating mutations involving exon 26 of NOTCH1. Of 24 individual cloned PCR products, 13 gave wild type sequences, 9 showed a mutation producing a L to P substitution in residue 1574, 1 showed the L1574P mutation in cis with a mutation producing a F to S substitution in residue 1592, and 1 showed the L1574P mutation in cis with a variant producing a L to P substitution in residue 1600. B) IHC staining for NICD1 in the same tumor, a formalin-fixed paraffin-embedded biopsy of a mediastinal mass. There is crush artifact, but strong nuclear staining is seen in intact cells.