Primary Cilia Are Lost in Preinvasive and Invasive Prostate Cancer (original) (raw)
Figure 2
Primary cilia frequency is decreased on both CK5+ and CK5- cancer cells.
Images of normal prostate and invasive prostate cancer. Nuclei (Hoechst; blue), cytokeratin 5 (CK5; white), primary cilia (Ac-Tub; red) and centrosomes (γ-Tub; green) are labeled. (A) CK5 marks the basal cell population in normal tissue. (A, left) A ciliated CK5+ normal cell and (A, right) an unciliated CK5+ cancer cell are shown at higher magnification. (B, left) A ciliated CK5- normal cell, and (B, right) an unciliated CK5- cancer cell. (C, top) Boxplots of the percent ciliated CK5+ and (D, top) CK5- epithelial and cancer cells per patient for each tissue type: normal, prostatic intraepithelial neoplasia (PIN), cancer (Ca), and perineural invasion (Peri). Q4, Q1 are as in Fig. 1. Statistics were performed using linear regression (*=p<0.05, ***= p<0.001). (C, bottom) Percent of CK5+ and (D, bottom) CK5- patients with an abnormally high percent cilia (Q4; orange), or an abnormally low percent cilia (Q1; blue).