Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein (original) (raw)
Figure 5
CXCR7 transduction does not affect surface expression of CXCR3 and CXCR4.
pLEC cultures were infected with Trans only or Trans+CXCR7 at indicated doses. At 20 hours post-infection cells were harvested and stained by flow cytometry for CXCR7 via 8F11 monoclonal antibody, CXCR4 and CXCR3. Necrotic cells were excluded from the analysis via propidium iodide staining and scatter characteristics. Positive staining (black line) was compared to appropriate isotype control (grey fill) and positive populations were gated based on isotype control levels. Data is representative of two experimental replicates.