Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein (original) (raw)
Figure 7
Expression of a CXCR7 mutant rescues Rb and prevents aberrant proliferation.
(A) pLEC cultures were transduced with CXCR7 WT at MOI 200 or CXCR7-Δ5 at MOI 50. At 20 hours post-infection cells were stained for surface expression of the HA tag. Propidium Iodide was used to exclude necrotic cells. (B) Total lysates from pLEC cultures transduced as in (A) were harvested and analyzed by western blot analysis for total Rb protein. (C) Confluent pLEC cultures transduced with increasing doses of CXCR7-WT (MOI 50, 100, 200 or 300) or CXCR7-Δ5 (MOI 10, 20, 30 or 50). At 20 hours post-infection, cells were lysed in the presence of Cyquant. Fluorescence values are normalized to Trans only controls. n = 10. * P = 0.03, ** P = 0.001.