Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome (original) (raw)
Figure 5
The alternative protein AltMRVI1 interacts with BRCA1.
(A) Sequence alignment between the third BRCA1 interacting protein 3 (BRCA1 IP3) identified by Liu et al by Y2H [40] and AltMRVI1. (B) Localization of BRCA1 and AltMRVI1EGFP in the nucleus. HeLa cells transfected with AltMRVI1EGFP (green channel) were immunostained with anti-BRCA1 (red channel) antibodies. Nuclei were stained with Hoechst (blue channel). Scale bar, 10 μm. (C) Co-immunoprecipitation with anti-GFP antibodies was performed using lysates from HeLa cells expressing AltMRVI1EGFP. Molecular weight markers in kDa are indicated on the right.