The Tip of the Tail Needle Affects the Rate of DNA Delivery by Bacteriophage P22 (original) (raw)
Figure 5
Potassium ion release by P22 phages with modified tail needles.
A. Infection of Salmonella strain UB-0001 by P22 phages with altered needle proteins at MOI = 10. Potassium ion release was measured at 30°C as described in Materials and methods with a potassium electrode. Salmonella host strain UB-0001 was infected by the following phages: UC-0911 fully P22 tail needle (▴); UC-0918, needle has Sf6 C-terminal knob domain and part of the shaft (▵); UC-0926, needle has HS1 C-terminal knob domain (•); UC-0927, foldon replaces needle C-terminal domain (⧫); no phage infection (○). B. Infection of Salmonella strain UB-0001 by P22 phages with foldon-tipped needle at various MOIs as follows: P22 UC-0911 with fully P22 tail needle at MOI = 10 (○); P22 UC-0927 where foldon replaces needle’s C-terminal domain at MOI = 10 (•), MOI = 50 (▪) and MOI = 100 (⧫); no phage (□). The horizontal axis is time after infection.