Type-Specific Cell Line Models for Type-Specific Ovarian Cancer Research (original) (raw)
Figure 1
Prediction of histotype was in part based on the COSP algorithm using 9 IHC markers [2].
(A–B) representative IHC from a typical high-grade serous ovarian carcinoma cell line, Kuramochi, and a clear cell carcinoma cell line, TOV21G. In addition to the 9-marker COSP panel, IHC for ARID1A (BAF250a) is also shown as a mutation surrogate. (C) TFF3 mRNA expression from 60 ovarian cancer samples (12 of each histotype). As noted previously high expression is most prevalent in MUC, followed by ENOCa and LGSC [2], [4]. Expression in our pilot cohort suggests the highest levels of TFF3 in MUC, which was significantly higher than all other groups (Tukey's adjusted p<0.01); no other pairwise comparisons had p<0.05. (D) TFF3 mRNA detected in ovarian cancer cell lines was used in place of an IHC score as the secreted TFF3 was considered a poor biomarker for cell culture conditions. Any cell line with measurable TFF3 mRNA above the NanoString detection threshold (see methods) was considered positive (score of 1 for use in the COSP algorithm).